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Image Search Results
Journal: The Journal of Neuroscience
Article Title: Spinal Wnt5a Plays a Key Role in Spinal Dendritic Spine Remodeling in Neuropathic and Inflammatory Pain Models and in the Proalgesic Effects of Peripheral Wnt3a
doi: 10.1523/JNEUROSCI.2942-19.2020
Figure Lengend Snippet: Noncanonical Ror2 and Ryk receptors mediate spinal Wnt5a-induced nociceptive hypersensitivity. A, B, Typical example of coimmunoreactivity for Ror2 (A) or Ryk (B; both in red) in the spinal dorsal horn, together with the markers for excitatory synapses (PSD-95, left) or inhibitory synapses (Gephyrin, right; both in green). Bottom panels show negative controls. Scale bar, 10 μm. C, D, Evidence for marked RNA interference-mediated knockdown of Ror2 (C) or Ryk (D) mRNA in the spinal dorsal horn on intrathecal injection of siRNA against Ror2 (si-Ror2) or Ryk (si-Ryk), respectively, in relation to intrathecal injection of the respective scrambled siRNAs as controls (si-Scramble). N= 4 mice/condition. Student's t test was performed. *p < 0.05 compared with the corresponding control group. E, H, Complete blockade of Wnt5a-induced mechanical hypersensitivity in mice intrathecally injected with siRNA against Ror2 (E, F) or siRNA against RyK (G, H) compared with mice injected with scrambled siRNA (n = 6–7 mice/group). Stimulus–response curves of the one paw are shown in E and G, whereas their integral over time (AUC) are shown in F and H. Two-way ANOVA followed by Tukey's test was performed. *p < 0.05 compared with the corresponding control group; †p < 0.05 compared with si-Scramble + Wnt5a-treated animals. In all panels, data are represented as the mean ± SEM, and bar graphs are also shown as scatter plots.
Article Snippet: Following Na-Citrate antigen retrieval treatment, the sections were stained with the following antibodies: sheep anti-Ryk (1:25; catalog #AF4649, R&D Systems), rabbit anti-Ror2 (1:100; catalog #PA5-77 028, Thermo Fisher Scientific), rabbit anti-PSD-95 (1:100; catalog #51–6900, Thermo Fisher Scientific),
Techniques: Injection